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June 29, 2011

“Otterly” Amazing Animals!

This past week, Cheyenne Mountain Zoo was fortunate enough to welcome, to the Rocky Mountain Wild exhibit, two new otters from the Minnesota Zoo in Apple Valley, MN. These two newbies helped increase our otter family to four meowters (aka males; females are called queens). A lot of zoo goers don’t know much about otters, so in honor of our new otter family members, we thought it would be a good time for us to share some of our otter knowledge. Plus “Otters” could be the subject of the final question on the next episode of Jeopardy and now you’ll know the answer…

According to early fossil remains, otters have been around for at least 30 million years. They can be found in very diverse locations close to bodies of water all over the world. “For $100 Alex – What animal lives all over the world, except in Australia and Antarctica?” Otters are considered a marine mammal, average about four feet in length, and can weigh up to 30 pounds. “For $200 Alex – What is the smallest marine mammal in the world?” Their tail can be up to a foot long and makes up about 40% of their body. An otter’s anatomy allows them to live both on land and in water. The little swimmers can remain under water for up to four minutes and they can dive up to 250 feet in search of food. Otters have the thickest fur of all fur animals, which means they are the only known marine animal that doesn’t have blubber and uses their fur to keep them warm. “For $300 Alex – What animal has at least 250,000 hairs per square inch?”

Here at Cheyenne Mountain Zoo, we are home to North American otters, but there are over 10 identified species of otters in the wild. In the wild, otters live for an average of 12 years. “For $400 Alex – What animal doubles their life span when in captivity?” They eat anything from fish to birds, and some people argue they also eat berries and leaves. Up to 25% of their body weight can be consumed in food on a daily basis. When female otters are nursing, they can eat even more than that. The families spend up to five hours a day hunting and consuming food. Like many otters, our otters here at Cheyenne Mountain Zoo are VERY playful and spend the majority of the day in the water or climbing the trees inside their exhibit. Otters love to sunbathe during the day and are very active at night as well. “For $500 Alex – What animal is considered to be one of the most intelligent animals in the world?”
  
Our four otters have become friends in a very short amount of time. We have Pahapi and Kuuyi who are four years old and have lived at our Zoo for the majority of their lives. The two new family members who were born just last year are Enapay, which means brave in Sioux, and Pivane, which means weasel in Hopi. The new additions are very dark in color and have been given the nickname dark chocolate. The older otters have been dubbed milk chocolate. We are so excited to have these two new additions!

Come up and visit them soon!   


June 20, 2011

Celebrating the 30th Anniversary of the Black-Footed Ferret’s Rediscovery - Part V: Breeding Season

The female black-footed ferrets (BFFs) come into estrus starting around March. They are checked weekly for signs of vulvar swelling. Once they are at a certain size, then vaginal washes are conducted. This allows for us to look at the epithelial cells to determine when she is at peak estrus. Samples are collected twice per week until she is ready for breeding.



The slides are stained using the Pap staining technique, which causes the cells to be different colors according to the level of estrus. In the early stages, the cells are round and have a very apparent nucleus. When she is near peak estrus, then the cells become more jagged, turn orange, and don’t have a nucleus. Once the female has 90% of the orange keratinized cells, then she is almost ready for breeding.

Peak Estrus
Early Estrus
An average years number of slides

The female is usually given 5-7 days to allow her to be at full estrus and then she is paired with a male. The males are selected using a Mate Suitability Index 1-6 (1 being excellent and 6 being very bad). This number comes from the inbreeding coefficient, mean kinship, and other genetic values of both animals. It is at this time when things start to get a little tricky. Our female is now ready, but what about the male? Is his sperm count above the 250 million per milliliter? Is he currently paired with a different female? Luckily our studbook keeper and genetic advisor have given optional backup pairing suggestions for when problems like this may occur, or if the male is busy being paired with a different female.

Once all is ready, the female is placed in the male’s enclosure. A camera is placed on the nest box to watch for breeding activity. These are normally solitary animals and if they are placed together at the wrong time, then one of them might be very aggressive toward the other. Usually our science has led us to the correct timing and the breeding goes smoothly. After about 30 minutes, the female is removed to do a vaginal wash. She is placed back quickly and the breeding continues. This wash is to check and see if there is sperm present. The challenge of this check is that there are often only a few sperm that are usually missing tails and there are a lot of other cells on the slide. It is almost like playing Where’s Waldo, but looking for a single sperm head. This can sometimes take over an hour of constant searching on the microscope. If there is no sperm found, then the female is re-paired but with a different male.

Power 10
Power 40


Digital Picture zoomed: a single sperm head

This step is important because BFFs females are induced ovulators. This means that the process of breeding will cause her to ovulate regardless of if there are sperm present. BFFs only come into estrus one time per year so if she ovulates and there are no sperm, then she may become pseudo pregnant. The female will then show all the signs of pregnancy and can even have a false birth and lactate, but no litter is produced. If this happens, then we have to wait an entire year before she can be bred again. BFFs are reproductive from the age of 1-4 for females. They only have 4 chances to reproduce, so we must make each year count.

June 13, 2011

Celebrating the 30th Anniversary of the Black-Footed Ferret’s Rediscovery - Part IV: Beginning of Breeding Season


Due to the Black-Footed Ferrets (BFFs) having a small founding population, it is important to maintain genetic diversity as much as possible. In order to do this, BFFs must be moved between breeding facilities so we are not repeatedly breeding the same pairs of ferrets year after year. The BFF Species Survival Plan (SSP) has a genetic advisor and studbook keeper, both use a computer program to decide what animals should be paired. Once these recommendations are made, the animals are transferred as needed. In order to reduce stress, we try to do as few transfers as possible. It also seems that some animals have better production if they are able to stay at the original facility they were raised at. However, we must do transfers to benefit the species in the long run. Most BFFs settle down fairly quickly due to all the breeding facilities having a similar setup and routine.

The lighting in the BFF room is a crucial factor for breeding success. The intensity is measured in foot-candles at each enclosure. Day length is also changed according to the time of year. Being from the Great Plains, their lighting needs are similar to our natural light cycle here in Colorado. However, we do adjust it each month going from 8 hours of light from Oct-Dec and increasing one hour each month until May. The increasing day length signals the BFFs to prepare for breeding season.

Male BFFs begin to show breeding readiness slightly earlier than the females. In late December, the testes start to enlarge and sperm production kicks into full gear. They are checked weekly for signs that they are coming into season. This is to ensure that they have sperm ready for when the females come into season later on. As a result of the genetic bottle neck, the sperm quantity and quality is decreased from what normal animals had in the wild prior to the bottleneck. In order to optimize breeding success, we conduct sperm concentration counts to make sure the males are at their prime breeding readiness. The males BFFs are anesthetized for the procedure and semen is collected. The samples that are collected are then loaded into a hemocytometer for counting under the microscope.


a) On each side of the hemocytometer is a square grid that contains 9 squares.


b) Only the 4 outside corners are counted, but on each side of the hemocytometer.


c) There is an even smaller grid within the grid on each side. In each corner, there
are 16 squares.



d) Each square is looked at and the sperm are counted in each of the 64 squares with
the average taken between the two sides. That number is then placed into an
equation to get the total concentration for the volume of semen collected.

Our goal is for the male to have above 250 million sperm per milliliter of semen. If the male is above the goal, then he can be used for breeding. If he is under, then he can be given a few more weeks and tested again. However, if the male is needed for a pairing before he is ready then he will be used if there are no other males available.

June 7, 2011

Celebrating the 30th Anniversary of the Black-Footed Ferret’s Rediscovery - Part III: Morning Duties


Black-footed ferrets (BFFs) are extremely susceptible to disease, so their home in Cheyenne Mountain Zoo’s Conservation Center is under strict quarantine. It takes a lot of work to keep up. For example, zookeepers shower before dressing in special clothes and shoes, which are worn only when caring for the ferrets. Latex gloves and face masks are also worn since BFFs, like the domestic ferret, can catch the human flu. These measures are important in preventing catastrophic events such as the plague and distemper outbreak that caused their original brush with extinction.

BFFs are solitary animals. Inside the Conservation Center, each BFF has their own upper enclosure with a tube leading down to a nest box, or burrow. The tube mimics a prairie dog tunnel. Mornings consist of cleaning and checking the health of each BFF. First, each BFF is moved from its upper enclosure to its lower nest box, and the upper enclosure is wiped down with a disinfectant. Next, the BFF is shifted out of their lower nest box to clean and disinfect the latrine side. Cleanliness is key for the BFFs, so each enclosure has its own cleaning supplies and nothing is ever used twice. After disinfecting the enclosure, clean Alpha-dri bedding is put down, which is a special compressed, highly-absorbent shredded paper. Multiply this process by 25 ferrets and you have a busy morning!

After about two hours, the BFFs are all back to sleep in their freshly cleaned nest boxes. We try to limit our time in the room as they are nocturnal, but go back in near the end of the day to feed and check on them. The BFFs get ground meat especially prepared for small zoo carnivores, and twice a week, a rodent. The bones from the rodents help keep their teeth clean and healthy. Since we are part of a reintroduction program, it is very important for their teeth to be in good health for hunting in the wild.



This breeding season (2011), we have nine males and 11 females. In addition, we care for five older BFFs unable to be released. They will eventually travel to other zoos or education facilities.

June 2, 2011

Farewell to an Old Friend: A Tribute to Elson



By Dina Bredahl, Animal Care Manager

Cheyenne Mountain Zoo experienced an irreplaceable loss Friday, May 27, 2011, when Elson, the zoo’s 16-year-old male African lion, passed away. Elson was receiving end of life care, and at the time of his death, was under anesthesia for treatment of a dental infection. It was during the procedure that Elson’s heart stopped. Typical life expectancy of African lions in captivity is 14 to 20 years.

Elson’s legacy is two-fold. He contributed critical founder genetics to the North American Lion Species Survival Plan (SSP) through his 13 cubs and at least seven grandcubs. These descendents live at Association of Zoos and Aquariums (AZA) facilities all across the U.S., including Abilene, Bronx, Pueblo, San Francisco, Minot, Tulsa, Akron, Baltimore, Birmingham, and here in Colorado Springs. He has also inspired the millions of zoo guests who have stared in awe at his grandeur. For many, it was the most unbelievable animal they had encountered in their life. What else made Elson so special? Read on for his story through the eyes and hearts of our staff.

Unlike most animals at Cheyenne Mountain Zoo, Elson was not born in a zoo. He was born in the wild at Kapama Reserve, located next to the Greater Kruger National Park in South Africa. To truly realize Elson’s significance, you have to understand the history of lions in zoos. Historically, when animals were imported in the early to mid 1900s, there was little documentation about their geographic origin. It was common practice to import lions from Asia and Africa, and allow them to reproduce. After many years of unmanaged breeding, many lions in U.S. zoos were “generic,” meaning no longer Asian or African, but rather a hybrid mix. In the 1990s, the SSP started working with reserves and biologists in South Africa to identify animals that could be exported without harming their conservation status in the wild. The SSP arranged for the importation of several lions from South Africa to establish a new population of the subspecies Panthera leo krugeri.


As you can imagine, the cost to import an African lion is quite a sum of money. One Cheyenne Mountain Zoo board member was extremely interested in the potential for Cheyenne Mountain Zoo to be involved in the critically important breeding of African lions, and volunteered to fund Elson’s importation. Elson arrived at Cheyenne Mountain Zoo in November of 1996. We know he was born in early 1995, so he was not quite two years old. He had the beginnings of a mane, with a spiky mohawk on his head and some unruly hair around his neck. Little did anyone know what a staggeringly beautiful animal he would become.

In April of 1997, Elson was introduced to Massina, a female who would serve as a short-term companion for Elson. It was in her company he developed into a mature lion. Massina was later sent to another zoo. Cheyenne Mountain Zoo was lucky enough to acquire a second wild-born African lion, a female named Angie. In December of 2000, Elson and Angie were introduced, and in June of 2002, Angie gave birth to a litter of four cubs. Elson had sired his first offspring!

When the cubs were 5 ½ weeks old, Elson had his first opportunity to enter the birthing den. He and Angie greeted each other, and Elson immediately went right over to smell and lick the cubs. When he tried to carry one outside, Angie put a stop to it. She would make the decision about when the cubs were ready, and Elson gracefully followed her lead. Together, Elson and Angie raised 13 cubs – a total of four litters, born in 2002, 2004, 2005, and 2007. These genetically valuable cubs were an important contribution to the African lion population in AZA-accredited zoos.

Elson was the most amazing father. He was gentle with the cubs when they were tiny, and they learned to respect his authority as they grew older. When each litter was old enough to come out and play, here was the typical scene - Elson would twitch the end of his tail and the cubs attacked it; Elson would playfully swat at the cubs, and they would run away into the bushes, preparing to stalk his tail again. The cubs would also jump on his back and roll clumsily off the other side. Elson’s patience was endless.

In another situation, Botswana, a male cub requiring around-the-clock care and bottle feedings from Cheyenne Mountain Zoo staff, was separated from the rest of his siblings for several weeks. To increase the chance of his acceptance, he was re-introduced to his litter mates first, so their smells would blend. Worst case scenario, Angie would reject the entire litter and refuse to nurse them, or Elson would smell the strange scents on the unfamiliar cub and attack him. Angie hissed a little when she first approached Botswana, but soon accepted him back into the litter and allowed him to nurse as if he had never left the lion building. Next, we gave Elson access to the birthing den and watched via camera. He batted at Botswana with his paws, as if he was testing him. Although Elson seemed a little suspicious for a few days, the play did not become too rough, and we all breathed a huge sigh of relief. This reaffirmed our belief Elson was a great father.

Elson might sound like the ideal lion, but there were some days he was less than perfect. Just as some house cats can act aloof and will only cooperate on their terms, so did the 400-pound wild African king of the savannah. On the average winter day, he was usually cooperative, however, the summer months were another story. His appetite slowed and he was often more interested in paying attention to Angie than listening to the animal keepers. It could feel like a major victory if Elson would decide to grace you with his presence and agree to shift into the off-exhibit holding building. Why did we care? The only way to properly clean a lion exhibit is to enter the exhibit, and in the case of large carnivores, the animals of course need to move elsewhere in order to do this. We tried all sorts of things, like rattling the feed chute door, dropping a bone loudly (this was his favorite treat), opening the meat packages in front of his door, and calling “El-son!” in the most convincing voice you could muster. Sometimes our little tricks worked, but often, he just lounged on his rock with his nose in the air. We all knew Elson’s wildness and uncompromising personality was a big part of what made him so unique and special.

One animal keeper states it well, calling Elson the “real deal.” He wanted to be out in the yard with his pride, making his territorial vocalizations. As he waited for Angie and the cubs to finish their training every day, he seemed to say, “Are you done playing with the humans yet?” He was never happy until his pride was back together and he was in control.

Here are more favorite memories from staff:

•Every morning and every evening like clockwork, no matter where you were in the zoo, you could hear Elson roar. The other lions usually joined him until it became a full chorus. It will not be the same without Elson’s impressive roar leading the pride.

•For those of us lucky enough to work with Elson on a daily basis, we sometimes heard him roaring territorially in the off-exhibit lion holding area. In an enclosed building like that, his roars echoed and were amplified, so you not only heard the vocalizations but felt them vibrate through your whole body. You couldn’t talk to fellow keepers, let alone hear yourself talk. You simply had to wait a minute or two while experiencing a true sense of wonder and exhilaration.

•We always had to place at least seven bones around the exhibit, even though there were only five lions. This is because it was rarely acceptable for Elson to end up with only one bone, even if it was the biggest. He took a big bone to his favorite spot, then stole a couple of bones from the other lions before hunkering down to enjoy his proper share. Everyone else knew better than to argue if he decided their bone looked like a good one.

•He looked so majestic eating meatballs, sometimes as large as his head. His rumble sounded like a noisy diesel engine.

•Elson got excited about scent enrichment like no other lion at Cheyenne Mountain Zoo. One of the lion keepers remembers spraying a new “manly” perfume on a big rock in the exhibit. When Elson went outside, he rubbed his face and mane all over that rock. He smelled like he had been out on a big date when he came back inside for his evening meal!

When any staff member from Cheyenne Mountain Zoo visits another zoo, we always find ourselves comparing every other male African lion to Elson. The conclusion is always the same - no other male comes close. He is truly the most beautiful male lion any of us have ever seen. I expect we will feel this way for the rest of our lives.